Adenosine uptake sites in rat brain: Identification using [3H]nitrobenzylthioinosine and co-localization with adenosine deaminase
نویسندگان
چکیده
منابع مشابه
Adenosine uptake sites in rat brain: identification using [3H]nitrobenzylthioinosine and co-localization with adenosine deaminase.
The binding characteristics of [3H]nitrobenzylthioinosine ([3H]NBI) to rat brain membrane preparations was examined, and the autoradiographic distribution of this ligand in brain sections was compared with the immunohistochemical localization of adenosine deaminase (ADA). It was found that [3H]NBI labels sites for which adenosine has far higher affinity than do other nucleosides, that these sit...
متن کاملAutoradiographic localization of adenosine uptake sites in rat brain using [3H]nitrobenzylthioinosine.
The adenosine uptake site has been localized in rat brain by an in vitro light microscopic autoradiographic method, using [3H]nitrobenzylthioinosine ( [3H]NBI) as the probe. The binding characteristics of [3H]NBI on slide-mounted sections are comparable to those seen in studies performed on brain homogenates. A very high density of uptake sites occurs in the nucleus tractus solitarius, in the s...
متن کاملLocalization of adenosine deaminase and adenosine deaminase complexing protein in rabbit heart. Implications for adenosine metabolism.
The distribution of adenosine deaminase and adenosine deaminase complexing protein in rabbit heart has been compared using immunohistochemical staining procedures. Sections (4-5 microns) of tissue fixed in Clarke's solution or paraformaldehyde and embedded in paraffin were stained by the peroxidase anti-peroxidase method for adenosine deaminase or complexing protein, using affinity purified ant...
متن کاملImmunohistochemical Localization of Adenosine Deaminase
Aim of Investigation: Four peptides, substance P (SP), somatostatin (SS), cholesystokinin (CCK) and vasoactive intestinal polypeptide (VIP) have been found to occur, and to some extent coexist, in small sensory gangion peurones in the cat. These cells have cutaneous (and other) receptors responding to noxious mechanical, heat, cold, and combinations of these modalities, and to non-noxious stimu...
متن کاملOpioid-induced decreases in rat brain adenosine levels are reversed by inhibiting adenosine deaminase.
BACKGROUND Opioids disrupt sleep and adenosine promotes sleep, but no studies have characterized the effects of opioids on adenosine levels in brain regions known to regulate states of arousal. Delivering opioids to the pontine reticular formation (PRF) and substantia innominata (SI) region of the basal forebrain disrupts sleep. In contrast, administering adenosine agonists to the PRF or SI inc...
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
ژورنال
عنوان ژورنال: Neuroscience Letters
سال: 1985
ISSN: 0304-3940
DOI: 10.1016/0304-3940(85)90310-6